大黄鱼EST微卫星标记初步筛选

福建水产 ›› 2011, Vol. 33 ›› Issue (5): 6-14.

大黄鱼EST微卫星标记初步筛选

谢芳靖¹,张子平²,邹志华¹,周鹏¹,王艺磊¹

1. 集美大学水产学院
2. 美国西东大学

收稿日期:2011-10-17 修回日期:2011-10-20 出版日期:2011-12-26 发布日期:2011-12-31
通讯作者: 王艺磊
基金资助:
福建省科技厅重点项目

A preliminary study on microsatellite markers screening from EST sequences of Larimichthys crocea

Received:2011-10-17 Revised:2011-10-20 Online:2011-12-26 Published:2011-12-31

摘要/Abstract

摘要： 通过构建大黄鱼性腺线性化cDNA文库，并经测序后获得3535条EST，对其二碱基至六碱基重复序列进行筛选，共发现微卫星位点150个，占EST序列的4.24%；其中包括二碱基重复序列64条，三碱基重复序列80条，四碱基重复序列5条，五碱基重复序列1条；三碱基重复序列是最丰富的重复单元，占53.3%。在这些微卫星序列中，(TG/GT/AC/CA)n形式在二碱基重复中最为常见，(GAG/AGG/GGA/CCT)n形式在三碱基重复序列中最为常见，分别占微卫星序列总数的26%和14%。选取其中的62条微卫星序列进行引物设计、合成与多态性检测，经过PCR扩增，2.0％的琼脂糖凝胶电泳检测，获得呈多态性微卫星引物8对，多态率为12.9％。本研究为开发大黄鱼EST微卫星分子标记和大黄鱼基因编码区微卫星的功能研究提供有价值的信息。

关键词: 大黄鱼, 表达序列标签, 微卫星, Larimichthys crocea, expressed sequence tag, microsatellite

Abstract: A normalized gonad cDNA library of Larimichthys crocea was constructed, from which 3535 EST were obtained by sequencing clones. The frequency and density of 2-6 bp SSR were analyzed from these ESTs. Amount of 150 SSR (4.24%) were obtained, among which there were 64 dinucleotide, 80 trinucleotide, 5 tetranucleotide and 1 pentanucleotide sequences. The trinucleotide repeats motifs were the most abundant motif in these SSR (53.3%). Among the dinucleotide and trinucleotide sequences(TG/GT/AC/CA)n and (GAG/AGG/GGA/CCT)n repeats were the dominant motifs, which accounted for 26% and 14%, respectively. 62 pairs of primers were designed from EST sequences containing SSR. And 8 of them showed polymorphic PCR products by electrophoresis on 2.0% agarose gel. Polymorphism rate was 12.9％. The results will contribute to the development of EST-SSR marker and the research of genes which contain SSR in coding regions.

中图分类号:

S917.4

谢芳靖张子平邹志华周鹏王艺磊. 大黄鱼EST微卫星标记初步筛选[J]. 福建水产, 2011, 33(5): 6-14.

[1]	葛辉吴丽云周宸黄种持吴建绍郑乐云林琪杨求华吴水清王艺磊林克冰. 斜带石斑鱼（Epinephelus coioides）病毒性神经坏死病毒的纯化分析及检测方法建立[J]. , 2019, 41(2): 96-105.
[2]	佟延南李高俊李芳远赵光军王德强. 海南省6个养殖罗非鱼群体遗传差异的微卫星分析[J]. 渔业研究, 2018, 40(1): 16-23.
[3]	吴文婵. 软质饲料与鲜杂鱼饵料饲养大黄鱼对比试验[J]. 福建水产, 2014, 36(3): 247-250.
[4]	吴靖娜刘智禹苏捷王茵许永安位绍红. GS-MS法分析液熏前后大黄鱼风味成分的变化[J]. , 2014, 36(2): 102-109.
[5]	何祥楷. 大黄鱼苗淀粉卵涡鞭虫病的诊断及防治方法[J]. , 2013, 35(6): 475-479.
[6]	刘振勇谢友佺林小金范希军. 感染刺激隐核虫的大黄鱼对低溶氧量的耐受力研究[J]. 福建水产, 2012, 34(6): 471-475.
[7]	李朝毅袁重桂阮成旭杨城. 大黄鱼幼鱼内陆可控生态精养技术初探[J]. 福建水产, 2012, 34(3): 236-239.
[8]	刘振勇. 大黄鱼刺激隐核虫病继发细菌感染致死原因的研究[J]. 福建水产, 2012, 34(1): 11-15.
[9]	刘家富韩坤煌. 我国大黄鱼产业的发展现状与对策[J]. 福建水产, 2011, 33(5): 4-8.
[10]	张善霹. 大黄鱼体内寄生纤毛虫病的诊治[J]. 福建水产, 2011, 33(2): 0-0.
[11]	胡荣炊. 大黄鱼的深水网箱养殖技术[J]. 福建水产, 2011, 33(2): 0-0.