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›› 2018, Vol. 40 ›› Issue (5): 374-379.

• 论文与报告 • 上一篇    下一篇

2个养殖水温下罗非鱼食后肠道消化酶活性的动态变化

翁祖桐   

  1. 福建省水产技术推广总站
  • 收稿日期:2018-08-06 修回日期:2018-08-25 出版日期:2018-10-25 发布日期:2018-11-01
  • 通讯作者: 翁祖桐
  • 基金资助:
    福建省科技计划重点项目;福建省海洋与渔业厅重点项目;福建省公益类科研院所专项

The variations of digestive enzyme activities after feeding in intestinal canal of tilapia at two water temperatures

weng zutong   

  • Received:2018-08-06 Revised:2018-08-25 Online:2018-10-25 Published:2018-11-01
  • Contact: weng zutong

摘要: This study was carried out to investigate the variation of digestive enzyme activities, including trypsin, amylase and lipase in anterior intestine, middle intestine and posterior intestine at 0, 1, 3, 6, 12, 24, 30, 36, 48, 56, 96 hours after feeding in Oreochromis niloticus at 26℃ and 30℃,respectively. The results showed that the enzyme activities of trypsin, amylase and lipase in anterior intestine, middle intestine and posterior intestine of tilapia had a tendency of ascending first and descending in succession after starvation and feeding. The enzyme activities at 30℃ were higher than those at 26℃. In addition, the tendency of ascending of enzyme activities at 30℃ appeared earlier and remained longer at high level than that at 26℃. The digestive enzyme activities presented variation with tissues selectivity in intestinal canal. The order of trypsin, amylase and lipase activities of different parts in intestinal canal were posterior intestine﹥anterior intestine﹥middle intestine, middle intestine﹥posterior intestine﹥anterior intestine ,and anterior intestine﹥middle intestine﹥posterior intestine,respectively. It was inferred that the main regions of digestive and absorption for protein, starch and fat element of tilapia feed in intestinal canal were posterior intestine, middle intestine and anterior intestine, respectively.

关键词: 养殖水温, “新吉富”罗非鱼, 肠道, 胰蛋白酶, 淀粉酶, 脂肪酶, water temperature, Oreochromis niloticus, intestinal canal, trypsin, amylase, lipase

Abstract: 在26 ℃和30 ℃养殖水温下,于“新吉富”罗非鱼摄食后0、1、3、6、12、24、30、36、48、56、96 h共11个时间点采样,分别研究了前、中、后肠胰蛋白酶、淀粉酶、脂肪酶活性的变化。试验结果显示,罗非鱼经饥饿再摄食后,前、中、后肠胰蛋白酶、淀粉酶、脂肪酶活性基本均呈现先上升再下降的趋势。在30 ℃养殖水温下,肠道各段消化酶活性均高于26 ℃养殖水温,且活性升高时间更早,较高活性水平持续时间更长。罗非鱼肠道各段消化酶活性存在组织差异性,在30 ℃养殖水温下,胰蛋白酶活性大小顺序为后肠﹥前肠﹥中肠,淀粉酶活性大小顺序为中肠﹥后肠﹥前肠,脂肪酶活性大小顺序为前肠﹥中肠﹥后肠,由此推测罗非鱼饲料中蛋白质、淀粉、脂肪成分在肠道中的主要消化部位分别为后肠、中肠、前肠。

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