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福建水产 ›› 2013, Vol. 35 ›› Issue (4): 290-295.

• 论文与报告 • 上一篇    下一篇

蜈蚣藻多糖提取工艺优化

张婧婧1,刘秋凤2,吴成业2   

  1. 1. 漳州科技学院
    2. 福建省水产研究所
  • 收稿日期:2013-05-17 修回日期:2013-07-06 出版日期:2013-08-23 发布日期:2013-10-30
  • 通讯作者: 吴成业

Optimization of Extraction Technology of Polysaccharides from Grateloupia filicina

  • Received:2013-05-17 Revised:2013-07-06 Online:2013-08-23 Published:2013-10-30
  • Contact: wu chengye

摘要: 以蜈蚣藻粗多糖产率为指标,比较分析了水提、碱提、酶解法及微波辅助等提取粗多糖的方法;以蜈蚣藻多糖得率为指标,研究了浸提温度、提取时间及固液比这三个因素对多糖得率的影响,并在单因素实验的基础之上,选取各因素的较优区域,进行三因素三水平的正交试验,确定了蜈蚣藻多糖提取工艺的最优组合。结果表明:热水浸提法提取蜈蚣藻多糖的产率最高,达到55.64%,且其最佳工艺为温度100℃、时间5 h、固液比1:60,按此工艺提取的蜈蚣藻多糖的得率为59.42%。

关键词: 蜈蚣藻, 多糖, 提取工艺, 正交试验, grateloupia filicina, polysaccharides, extraction technology, orthogonal experiment

Abstract: Using the yield of crude polysaccharides from grateloupia filicin as index, this thesis compared and analysed several extraction methods, including water extraction, alkali extraction, enzymatic hydrolysis and microwave-assisted extraction. Using the yield of polysaccharides from grateloupia filicin as index, this thesis studied the factors that influence the index, including treatment temperature, time and S/L ratio,through the single-factor experiment and the orthogonal test, the optiamal cripness of extraction technology of polysaccharides was established. The results indicated that water extraction was the best method to extract polysaccharides and the extraction efficiency was 55.64%t, the optimal extraction technology conditions were determined to temperature of 100℃,time of 5h,solid-liquid ratio of 1:60,the yield of polysaccharides up to 59.42% .

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